Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds
Identifieur interne : 009C82 ( Main/Exploration ); précédent : 009C81; suivant : 009C83Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds
Auteurs : Richard M. Knuckey [Australie] ; Malcolm R. Brown [Australie] ; René Robert [France] ; Dion M. F. Frampton [Australie]Source :
- Aquacultural engineering [ 0144-8609 ] ; 2006.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Aquiculture.
English descriptors
- KwdEn :
Abstract
A novel technique was developed for the flocculation of marine microalgae commonly used in aquaculture. The process entailed an adjustment of pH of culture to between 10 and 10.6 using NaOH, followed by addition of a non-ionic polymer Magnafloc LT-25 to a final concentration of 0.5 mg L-1. The ensuing flocculate was harvested, and neutralised giving a final concentration factor of between 200- and 800-fold. This process was successfully applied to harvest cells of Chaetoceros calcitrans, C. muelleri, Thalassiosira pseudonana, Attheya septentrionalis, Nitzschia closterium, Skeletonema sp., Tetraselmis suecica and Rhodomonas salina, with efficiencies >80%. The process was rapid, simple and inexpensive, and relatively cost neutral with increasing volume (cf. concentration by centrifugation). Harvested material was readily disaggregated to single cell suspensions by dilution in seawater and mild agitation. Microscopic examination of the cells showed them to be indistinguishable from corresponding non-flocculated cells. Chlorophyll analysis of concentrates prepared from cultures of ≤130 L showed minimal degradation after 2 weeks storage. Concentrates of T. pseudonana prepared using pH-induced flocculation gave better growth of juvenile Pacific oysters (Crassostrea gigas) than concentrates prepared by ferric flocculation, or centrifuged concentrates using a cream separator or laboratory centrifuge. In follow up experiments, concentrates prepared from 1000 L Chaetoceros muelleri cultures were effective as supplementary diets to improve the growth of juvenile C. gigas and the scallop Pectenfumatus reared under commercial conditions, though not as effective as the corresponding live algae. The experiments demonstrated a proof-of-concept for a commercial application of concentrates prepared by flocculation, especially for use at a remote nursery without on-site mass-algal culture facilities.
Affiliations:
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Brown</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>CSIRO Marine Research, GPO Box 1538 Hobart</s1><s2>Tasmania 7001</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Tasmania 7001</wicri:noRegion></affiliation></author><author><name sortKey="Robert, Rene" sort="Robert, Rene" uniqKey="Robert R" first="René" last="Robert">René Robert</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>IFREMER, UMR Physiologie et Ecophysiologie des Mollusques Marins Station Expérimentale d'Argenton Presqu'île du Vivier</s1><s2>29840 Argenton en Landunvez</s2><s3>FRA</s3><sZ>3 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>29840 Argenton en Landunvez</wicri:noRegion><wicri:noRegion>UMR Physiologie et Ecophysiologie des Mollusques Marins Station Expérimentale d'Argenton Presqu'île du Vivier</wicri:noRegion><wicri:noRegion>29840 Argenton en Landunvez</wicri:noRegion></affiliation></author><author><name sortKey="Frampton, Dion M F" sort="Frampton, Dion M F" uniqKey="Frampton D" first="Dion M. 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Knuckey</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Primary Industries and Fisheries, Northern Fisheries Centre, GPO Box 5396</s1><s2>Cairns, Qld 4870</s2><s3>AUS</s3><sZ>1 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Cairns, Qld 4870</wicri:noRegion></affiliation></author><author><name sortKey="Brown, Malcolm R" sort="Brown, Malcolm R" uniqKey="Brown M" first="Malcolm R." last="Brown">Malcolm R. Brown</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>CSIRO Marine Research, GPO Box 1538 Hobart</s1><s2>Tasmania 7001</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Tasmania 7001</wicri:noRegion></affiliation></author><author><name sortKey="Robert, Rene" sort="Robert, Rene" uniqKey="Robert R" first="René" last="Robert">René Robert</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>IFREMER, UMR Physiologie et Ecophysiologie des Mollusques Marins Station Expérimentale d'Argenton Presqu'île du Vivier</s1><s2>29840 Argenton en Landunvez</s2><s3>FRA</s3><sZ>3 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>29840 Argenton en Landunvez</wicri:noRegion><wicri:noRegion>UMR Physiologie et Ecophysiologie des Mollusques Marins Station Expérimentale d'Argenton Presqu'île du Vivier</wicri:noRegion><wicri:noRegion>29840 Argenton en Landunvez</wicri:noRegion></affiliation></author><author><name sortKey="Frampton, Dion M F" sort="Frampton, Dion M F" uniqKey="Frampton D" first="Dion M. F." last="Frampton">Dion M. F. Frampton</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>CSIRO Marine Research, GPO Box 1538 Hobart</s1><s2>Tasmania 7001</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Tasmania 7001</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Aquacultural engineering</title><title level="j" type="abbreviated">Aquac. eng.</title><idno type="ISSN">0144-8609</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Aquacultural engineering</title><title level="j" type="abbreviated">Aquac. eng.</title><idno type="ISSN">0144-8609</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aquaculture</term><term>Bivalvia</term><term>Environmental engineering</term><term>Flocculation</term><term>Paste</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Floculation</term><term>Aquiculture</term><term>Pâte</term><term>Ingénierie environnement</term><term>Bivalvia</term><term>Thalassiosira</term><term>Chaetoceros</term><term>Microalgue</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Aquiculture</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel technique was developed for the flocculation of marine microalgae commonly used in aquaculture. The process entailed an adjustment of pH of culture to between 10 and 10.6 using NaOH, followed by addition of a non-ionic polymer Magnafloc LT-25 to a final concentration of 0.5 mg L<sup>-1</sup>. The ensuing flocculate was harvested, and neutralised giving a final concentration factor of between 200- and 800-fold. This process was successfully applied to harvest cells of Chaetoceros calcitrans, C. muelleri, Thalassiosira pseudonana, Attheya septentrionalis, Nitzschia closterium, Skeletonema sp., Tetraselmis suecica and Rhodomonas salina, with efficiencies >80%. The process was rapid, simple and inexpensive, and relatively cost neutral with increasing volume (cf. concentration by centrifugation). Harvested material was readily disaggregated to single cell suspensions by dilution in seawater and mild agitation. Microscopic examination of the cells showed them to be indistinguishable from corresponding non-flocculated cells. Chlorophyll analysis of concentrates prepared from cultures of ≤130 L showed minimal degradation after 2 weeks storage. Concentrates of T. pseudonana prepared using pH-induced flocculation gave better growth of juvenile Pacific oysters (Crassostrea gigas) than concentrates prepared by ferric flocculation, or centrifuged concentrates using a cream separator or laboratory centrifuge. In follow up experiments, concentrates prepared from 1000 L Chaetoceros muelleri cultures were effective as supplementary diets to improve the growth of juvenile C. gigas and the scallop Pectenfumatus reared under commercial conditions, though not as effective as the corresponding live algae. The experiments demonstrated a proof-of-concept for a commercial application of concentrates prepared by flocculation, especially for use at a remote nursery without on-site mass-algal culture facilities.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Knuckey, Richard M" sort="Knuckey, Richard M" uniqKey="Knuckey R" first="Richard M." last="Knuckey">Richard M. Knuckey</name></noRegion><name sortKey="Brown, Malcolm R" sort="Brown, Malcolm R" uniqKey="Brown M" first="Malcolm R." last="Brown">Malcolm R. Brown</name><name sortKey="Frampton, Dion M F" sort="Frampton, Dion M F" uniqKey="Frampton D" first="Dion M. F." last="Frampton">Dion M. F. Frampton</name></country><country name="France"><noRegion><name sortKey="Robert, Rene" sort="Robert, Rene" uniqKey="Robert R" first="René" last="Robert">René Robert</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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